Plasmid_Backbone

Part:BBa_K4404007

Designed by: Julia Fricke   Group: iGEM22_Goettingen   (2022-10-09)


pMTL83151

pMTL83151 is based on the modular plasmid system ClosTron (Heap et al., 2009) with the Gram-positive replicon pCB102 and ColE1 as a Gram-negative replicon. traJ facilitates the transfer of the plasmid and the catP cassette serves as an antibiotic (chloramphenicol) selection marker. lacZ codes for β-galactosidase, sits at the multiple cloning site and serves as the transformational control.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 375
    Illegal EcoRI site found at 3850
    Illegal XbaI site found at 3877
    Illegal PstI site found at 3740
    Illegal PstI site found at 3916
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 375
    Illegal EcoRI site found at 3850
    Illegal NheI site found at 4095
    Illegal PstI site found at 3740
    Illegal PstI site found at 3916
    Illegal NotI site found at 3819
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 375
    Illegal EcoRI site found at 3850
    Illegal BglII site found at 3904
    Illegal BamHI site found at 3871
    Illegal XhoI site found at 3908
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 375
    Illegal EcoRI site found at 3850
    Illegal XbaI site found at 3877
    Illegal PstI site found at 3740
    Illegal PstI site found at 3916
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 375
    Illegal EcoRI site found at 3850
    Illegal XbaI site found at 3877
    Illegal PstI site found at 3740
    Illegal PstI site found at 3916
    Illegal NgoMIV site found at 1289
    Illegal NgoMIV site found at 3196
    Illegal NgoMIV site found at 3206
    Illegal NgoMIV site found at 3332
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2948
    Illegal SapI.rc site found at 3303
    Illegal SapI.rc site found at 3674


[edit]
Categories
Parameters
None